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KMID : 0616120070380040349
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Korean Journal of Pharmacognosy
2007 Volume.38 No. 4 p.349 ~ p.353
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Effect of Torilis Fructus on Procollagen Biosynthesis and Activity of Matrix Metalloproteinase-I(MMP-1) in Human Dermal Fibroblast
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Koo Bon-Suk
Hwang Eui-Il
So Seung-Ho
Lee Seong-Kye
Han Gyeong-Ho
Kim Na-Mi
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Abstract
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Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase-I(MMP-1) activity. This study was carried out to find out skin wrinkle reducing components in Torilis Fructus. Torilis Fructus were extracted with 70% ethanol and the ethanol extracts were systematically fractionated with n-hexane, ethylacetate, n-butanol and distilled water. Among them, antiwrinkle component from n-hexane fraction was purified by several column chromatographies and HPLC, which identified as torilin by 1 H-NMR, 13 C-NMR and ESI -MS. To determine cell viability, collagen biosynthesis and MMP-1 activity, human dermal fibroblast was treated with 1-5 ppm concentrations of Torilis Fructus extract fraction and torilin. Cell viability was showed 84-102% at all group treated with 1-5 ppm. Collagen synthesis was increased in all group, especially torilin-treated group was highest amount. Active forms of MMP-1 were decreased in all group. From these results, we consider that Torilis Fructus have several antiwrinkle components and torilin may be one of the effective components.
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KEYWORD
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human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I, torilin, Torilis Fructus
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